Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects

2001 ◽  
Vol 21 (2) ◽  
pp. 109-119 ◽  
Author(s):  
G.D. Castro ◽  
A.M.A. Delgado de Lay�o ◽  
M.H. Costantini ◽  
J.A. Castro
Keyword(s):  
Free Radicals ◽  
Xanthine Oxidase ◽  
Alcohol Drinking ◽  
Potential Role ◽  
Ventral Prostate ◽  
Heavy Alcohol ◽  
Rat Ventral Prostate

Rat ventral prostate xanthine oxidase–mediated metabolism of acetaldehyde to acetyl radical

2009 ◽  
Vol 28 (4) ◽  
pp. 203-208 ◽  
Author(s):  
GD Castro ◽  
MH Costantini ◽  
JA Castro
Keyword(s):  
Epithelial Cells ◽  
Xanthine Oxidase ◽  
Alcohol Drinking ◽  
Metabolic Pathways ◽  
Ventral Prostate ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Low Levels ◽  
Acetyl Radical ◽  
And Oxidative Stress

Alcohol drinking is known to lead to deleterious effects on prostate epithelial cells from humans and experimental animals. The understanding of the mechanisms underlying these effects is relevant to intraprostatic ethanol treatment of benign prostatic hyperplasia and to shed some light into the conflictive results linking alcohol consumption to prostate cancer. In previous studies, we provided evidence about the presence in the rat ventral prostate of cytosolic and microsomal metabolic pathways of ethanol to acetaldehyde and 1-hydroxyethyl radical and about the low levels of alcohol dehydrogenase and aldehyde dehydrogenase. Acetaldehyde accumulation in prostate tissue and oxidative stress promotion were also observed. In this study, we report that in the ventral prostate cytosolic fraction, xanthine oxidoreductase is able to metabolize acetaldehyde to acetyl radical. The identification of the acetyl was performed by GC-MS of the silylated acetyl-PBN adduct. Reference adduct was generated chemically. Formation of acetyl was also observed using pure xanthine oxidase. The generation of acetyl by the prostate cytosol was inhibited by allopurinol, oxypurinol, diphenyleneiodonium chloride, folate, and ellagic acid. Results suggest that metabolism of ethanol to acetaldehyde and to 1-hydroxyethyl and acetyl radicals could be involved in the deleterious effects of alcohol drinking on prostate epithelial cells.

Biochemical and ultrastructural alterations in the rat ventral prostate due to repetitive alcohol drinking

2007 ◽  
Vol 27 (4) ◽  
pp. 391-398 ◽  
Author(s):  
M. I. Díaz Gómez ◽  
C. Rodríguez de Castro ◽  
S. L. Fanelli ◽  
L. N. Quintans ◽  
M. H. Costantini ◽  
...  
Keyword(s):  
Alcohol Drinking ◽  
Ventral Prostate ◽  
Ultrastructural Alterations ◽  
Rat Ventral Prostate

Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate

1987 ◽  
Vol 147 (1) ◽  
pp. 196-203 ◽  
Author(s):  
Jocelyne G. Léger ◽  
Michael L. Montpetit ◽  
Martin P. Tenniswood
Keyword(s):  
Ventral Prostate ◽  
Rat Ventral Prostate

FURTHER STUDIES ON A SOLUBLE ANDROGEN-MACROMOLECULAR COMPLEX FROM THE RAT VENTRAL PROSTATE

1970 ◽  
Vol 65 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Olav Unhjem
Keyword(s):  
Hydrogen Atom ◽  
Keto Group ◽  
Ventral Prostate ◽  
Metabolic Inhibitors ◽  
Macromolecular Complex ◽  
Structural Requirements ◽  
Macromolecular Complexes ◽  
Rat Ventral Prostate

ABSTRACT The ability of various steroids and metabolic inhibitors to influence the binding of androgen to soluble macromolecules in the rat ventral prostate was evaluated in vitro. The results obtained revealed some structural requirements of steroids for binding to the macromolecules. An androstane skeleton with the α-configuration of the hydrogen atom at position 5 seemed to be essential for binding as well as a keto group at position 3. N-ethylmaleimide, Na-iodoacetate and p-hydroxymercuribenzoate inhibited the binding of androgen to macromolecules. The androgen-macromolecular complexes appeared to be rather stable at temperatures below 5°C.

Ca2+-activated Cl? channel currents in rat ventral prostate epithelial cells

The Prostate ◽  
2003 ◽  
Vol 55 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Sung Joon Kim ◽  
Sun Young Shin ◽  
Ji Eun Lee ◽  
Jun Hee Kim ◽  
Dae-Yong Uhm
Keyword(s):  
Epithelial Cells ◽  
Ventral Prostate ◽  
Cl Channel ◽  
Prostate Epithelial Cells ◽  
Rat Ventral Prostate ◽  
Channel Currents
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